Interleukin-6 Responses in Red Sokoto Bucks Following Orchidectomy, Burdizzo Castration and In-situ Spermatic Cord Ligation

Terfa Ornande Nev; Ochuko Orakpoghenor; Ashever Jocelyn Terfa

doi:10.5455/JRVS.20240120104728

Abstract
Aim:This study aimed to evaluate the effects of orchidectomy, Burdizzo castration, and in-situ spermatic cord ligation on interleukin 6 (IL-6) responses in red Sokoto bucks.
Methods:Sixteen (16) Sokoto red bucks (6 months to 1 year old and 11-12 kg body weight) were randomly assigned and divided into 4 groups (A, B, C, and D) each containing 4 bucks. Group A were castrated using Burdizzo), B in-situ spermatic cord ligation, and C orchidectomy while group D (control) was not castrated. Blood was collected pre-castration, at immediate, 4, 8, 12, 16, 20, 24-, 32-, 48- and 72-hours post-castration (hpc) into plain labeled tubes, serum harvested and analyzed for IL-6 levels.
Results:Results revealed significant (p < 0.05) increased IL-6 levels in groups A, B, and C from 4 hpc, reaching peak levels at 20 hpc with the highest recorded in group C followed by group B and then group A.
Conclusion:In conclusion, orchidectomy, Burdizzo castration, and in-situ spermatic cord ligation elicited IL-6 responses but the response was highest with orchidectomy followed by in-situ spermatic cord ligation,then Burdizzo castration. This indicates that these castration methods increased inflammation, thus suggesting that IL-6 can be used to determine inflammatory responses for bucks after castration. This is the first study to demonstrate a relationship between orchidectomy, Burdizzo castration in-situ spermatic cord ligation, and serum IL-6 levels in the red Sokoto bucks

Key words: orchidectomy; inflammation; Burdizzo castration; interleukin-6; goat; in-situ spermatic cord ligation