E-ISSN 2983-757X
 
2025, Vol: 5, Issue: 2
5 / 2Current Issue Online FirstArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles

Original Article
Online Published: 08 Aug 2024
 


Transferrin binds zinc at a site different from the iron-binding site

Michio Katagi, Rintaro Seki, Yasunaga Yoshikawa, Taisuke Kitano, Koichi Orino.


Abstract
Background: Bovine apotransferrin strongly binds beads immobilized with zinc ion, indicating that transferrin carry zinc ion as well as iron.
Aim: The aim of this study is to elucidate the binding mechanism between zinc ion and transferrin, because, if zinc ion binding site is same binding site with iron, zinc ion competes with iron in the same binding site.
Methods: This study prepared beads which was immobilized with apotransferrin or zinc ion for the binding analysis of bovine transferrin with zinc ion. After binding of bovine apotransferrin with zinc-ion immobilized beads, the binding of apotransferrin with iron was examined.
Results: Beads immobilized with bovine apo-transferrin showed significantly lower zinc recovery compared with control beads that showed almost complete recovery of zinc ions derived from zinc sulfate added to each bead solution. Bovine apo- and holo-transferrin bound to zinc ion–immobilized beads, and holo-transferrin still bound iron even when binding to the beads. Zinc ion-binding apo-transferritin incorporated iron in the presence of ferrous ammonium sulfate and horse spleen apoferritin with ferroxidase.
Conclusion: These results suggest that transferrin directly binds zinc ions at sites other than iron-binding sites.

Key words: apo-transferrin, ferroxidase, holo-transferrin, iron, zinc


 
ARTICLE TOOLS
Abstract
PDF Fulltext
HTML Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by MIchio Katagi
Articles by Rintaro Seki
Articles by Yasunaga Yoshikawa
Articles by Taisuke Kitano
Articles by Koichi Orino
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Katagi M, Seki R, Yoshikawa Y, Kitano T, Orino K. Transferrin binds zinc at a site different from the iron-binding site. J Res Vet Sci. 2024; 3(3): 98-102. doi:10.5455/JRVS.20240724010345

Web Style

Katagi M, Seki R, Yoshikawa Y, Kitano T, Orino K. Transferrin binds zinc at a site different from the iron-binding site. https://www.wisdomgale.com/jrvs/?mno=211166 [Access: March 31, 2025]. doi:10.5455/JRVS.20240724010345

AMA (American Medical Association) Style

Katagi M, Seki R, Yoshikawa Y, Kitano T, Orino K. Transferrin binds zinc at a site different from the iron-binding site. J Res Vet Sci. 2024; 3(3): 98-102. doi:10.5455/JRVS.20240724010345


Vancouver/ICMJE Style

Katagi M, Seki R, Yoshikawa Y, Kitano T, Orino K. Transferrin binds zinc at a site different from the iron-binding site. J Res Vet Sci. (2024), [cited March 31, 2025]; 3(3): 98-102. doi:10.5455/JRVS.20240724010345


Harvard Style

Katagi, M., Seki, . R., Yoshikawa, . Y., Kitano, . T. & Orino, . K. (2024) Transferrin binds zinc at a site different from the iron-binding site. J Res Vet Sci, 3 (3), 98-102. doi:10.5455/JRVS.20240724010345


Turabian Style

Katagi, Michio, Rintaro Seki, Yasunaga Yoshikawa, Taisuke Kitano, and Koichi Orino. 2024. Transferrin binds zinc at a site different from the iron-binding site. Journal of Research in Veterinary Sciences, 3 (3), 98-102. doi:10.5455/JRVS.20240724010345


Chicago Style

Katagi, Michio, Rintaro Seki, Yasunaga Yoshikawa, Taisuke Kitano, and Koichi Orino. "Transferrin binds zinc at a site different from the iron-binding site." Journal of Research in Veterinary Sciences 3 (2024), 98-102. doi:10.5455/JRVS.20240724010345


MLA (The Modern Language Association) Style

Katagi, Michio, Rintaro Seki, Yasunaga Yoshikawa, Taisuke Kitano, and Koichi Orino. "Transferrin binds zinc at a site different from the iron-binding site." Journal of Research in Veterinary Sciences 3.3 (2024), 98-102. Print. doi:10.5455/JRVS.20240724010345


APA (American Psychological Association) Style

Katagi, M., Seki, . R., Yoshikawa, . Y., Kitano, . T. & Orino, . K. (2024) Transferrin binds zinc at a site different from the iron-binding site. Journal of Research in Veterinary Sciences, 3 (3), 98-102. doi:10.5455/JRVS.20240724010345


Author LoginReviewer LoginAbout PublisherOpen Access PolicyEditorial PoliciesEditorial Review PolicyPeer Review PolicyEditorial & Peer Review ProcessPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyProtection of Research Participants (Statement On Human And Animal Rights)Privacy PolicyCorrections, Retractions & Expressions of ConcernSelf-Archiving PoliciesDigital Archiving & Preservation PoliciesTerms of UseLicense InformationCopyright Information

About Journal of Research in Veterinary Sciences

Journal of Research in Veterinary Sciences is an international, peer-reviewed, open access journal, providing a platform for advances in basic, translatio ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

Your comments are very important to us

We welcome your ideas, suggestions, comments, or questions. To reach Editorial Board please use one of the methods provided below.
 

Emails:

info@wisdomgale.com
editorinchief@ejmanager.com

Office Address:
WisdomGale Publishing
14 Rue de Grand-Bigard,
1082 Brussels
Belgium
 
We will respond within 48 to 72 hours.
 
Change of address or personal information? Please visit your account at www.ejmanager.com to update your information.