Clinicopathological and hematological profile of silver rasbora (Rasbora argyrotaenia) after experimentally infection with Aeromonas hydrophila

Aulia Azizah; Mohammad Faizal Ulkhaq; Dian Putri Apriliani; Wahju Tjahjaningsih; Annur Ahadi Abdillah

doi:10.5455/JRVS.20230811124754

Abstract
Aim: The study aims to analyse clinicopathological changes and hematology profiles for early detection in silver rasbora infected by A. hydrophila.
Methods: This study was conducted in Januari-Februari 2022. The treatment used in this study was experimental infection with A. hydrophila intramuscularly in silver rasbora using several concentrations, including 104 (P1), 106 (P2), 108 (P3), 1010 (P4) CFUmL-1, and a negative control (P0) (injected with normal saline) and reared for two days post infection. The research data focused on histopatological alteration in the liver and kidney and the hematological profile of infected fish. The mortality data and histopathology organ were analyzed using one-way ANOVA. Furthermore, if a significant value was detected, further test would be carried out using the Duncan Multiple Range test and Mann-Whitney test, respectively. Other parameters, including hematological profile and clinical signs, were analyzed descriptively.
Results: Infected silver rasbora (R. argyrotaenia) showed clinical signs, consisting of behavioural changes, external lesions, a hematological profile, and histopathological changes, in the kidney and liver. An increase in the density of A. hydrophila infected in silver rasbora caused severe changes in the clinicopathological characteristics of liver and kidney cells, consisting of degeneration and necrosis. The result presented significant hematology changes, including an increase in neutrophil and monocyte percentages. In contrast, decreasing total erythrocyte, hemoglobin level, total leucocyte, and lymphocyte percentages were observed.
Conclusion: The silver rasbora infected by 1010 CFUmL-1 of A. hydrophila exhibited the lowest survival rate (SR), significant changes in haematology profiles, and severe histological damage.

Key words: Aeromonas hydrophila, Blood parameters, Experimental infection, Histopathological changes, Rasbora argyrotaenia