Clinico-pathological changes and performance indices in Eimeria tenella-infected broiler chickens following the prophylactic administration of Ganoderma lucidum, Vernonia amygdalina, and Vitellaria paradoxa methanolic extract

Paul Terkende Hambesha; Ochuko Orakpoghenor; Paul Ayuba Abdu; Jatau Isa Danladi; Tagang Aluwong

doi:10.5455/JRVS.20230818042509

Abstract
This study assessed the clinico-pathological changes and performance indices in Eimeria tenella-infected broiler chickens following the prophylactic administration of methanol extracts of Ganoderma lucidum, Vernonia amygdalina and Vitellaria paradoxa. One hundred one-day-old Abor acre broiler chicks were randomly divided into 10 groups (A1, B1, C1, D1, A2, B2, C2, D2, E, and F) of 10 birds each. From 14-20 days of age (doa), groups A1 and A2 were administered G. lucidum; B1 and B2 V. amygdalina; C1 and C2 V. paradoxa methanol extracts; D1 and D2 Amprolium; while groups E and F were not administered any extract/medication. At 21 doa, groups A2, B2, C2, D2 and E were challenged with 104 E. tenella sporulated oocysts orally. Clinical signs, morbidity and mortality rates, caecal lesion scores and performance indices were determined. Outcomes revealed mild clinical signs in groups A2, B2, and C2, moderate in D2 but severe in E; 100% morbidity and 0.0% mortality in all groups of chickens. Caecal lesions were mild in groups A2, B2, C2 and D. There was non-significantly (P > 0.05) higher weight gain and lower feed conversion ratio in groups A2, B2, C2 and D2 compared to E. The oocyst decrease ratio and anticoccidial index were higher in A2, B2, C2 and D2 than E. The prophylactic administration of G. lucidum, V. amygdalina leaf and V. paradoxa stem bark methanol extracts decreased the clinical signs, caecal lesions, and histopathological changes, and improved performance in the E. tenella-challenged broiler chickens. The identification of the bioactive substances in these extracts responsible for their prophylactic anticoccidial activities and their possible mechanisms is therefore recommended.

Key words: methanol extract, prophylactic, anticoocidial, clinico-pathological changes, performance