ACUTE TOXICITY AND ANTIDIABETIC ACTIVITY OF PHYLLANTHUS NIRURI USING ZEBRA FISH

Alekkhya Veeramaneni; Deepan Thiyagarajan; Sandhya Reddy; Mookambika Mattateja; Koteswararao Nerella; Dhanaraju Dasaratha Magharla

doi:10.5455/JBRR.20240801045925

Abstract
This study was designed to assess the acute toxicity and antidiabetic activity of Ethanolic extract of Phyllanthus niruri (EEPN). Several invivo studies have demonstrated that extracts of Phyllanthus niruri has significantly lowered the blood glucose levels and lowers the complications associated with Type 2 diabetes in rodents, but till date no studies have been reported using zebrafish model for antidiabetic activity using Phyllanthus niruri. In the present study zebra fish are divided into six groups and given doses of 6, 12, 25, 50, 100, 200, and 400 mg/L of EEPN for extraction. These fish are then examined at intervals of 6, 24, 48, and 72 hours.Thirty zebra fish were divided into control and treatment groups for the purpose of evaluating their anti-diabetic activity. For 30 days, high glucose diet was used to induce diabetes in all zebra fish. The test groups are treated with concentrations of the extracts 25mg/L & 50mg/L and standard with 20mM metformin. The blood glucose levels are estimated in alternative days for seven days and on seventh day fish were studied histopathologically. Acute toxicity study of EEPN does not show any mortality,morbidity, and behavioural changes even after 96 hrs at maximum concentration 400mg/L. The extract showed significant lowering of blood glucose levels on diabetic zebra models. Extensive study can be done on the extract to study the natural lead compounds from this plant which are responsible for antidiabetic activity.

Key words: Acute toxicity, Antidiabetic activity, Phyllanthus niruri, Zebrafish