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2025, Vol: 2, Issue: 3
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Original Article
Online Published: 18 Jun 2025
 


THE EFFECT OF METHANOL EXTRACT OF Sabicea brevipes (RUBIACEAE) ON SELECTED HAEMATOLOGICAL PARAMETERS IN NORMAL HEALTHY WISTAR RATS

Gladys Ndidiamaka Ugwu, Chimaobi Odinaka Ugorji, Paul Chinwuba, Chioma Obiageli Onyesife, Vivian Ifeoma Okonkwo, Favour Chidiebere Edeh, Abigail Oluchi Odo.


Abstract
Abstract
Background: Sabicea brevipes, a shrub of the Rubiaceae family, has been used for medicinal benefits. We examined the phytochemical components, acute toxicity, and impact of the methanol extract of Sabicea brevipes leaves on blood parameters in healthy wistar rats.
Method: Qualitative and quantitative phytochemical analyses were performed to detect and quantify bioactive compounds. Acute toxicity testing showed no toxic effects or fatalities at the highest dose (5000 mg/kg), indicating a broad safety margin. Wistar rats received oral doses of the extract at 100, 200, and 400 mg/kg for 21 days, with distilled water as control. Blood parameters assessed included red blood cell (RBC) count, haemoglobin (Hb) concentration, packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), white blood cell (WBC) count, and differential leukocyte count.
Results: The extract significantly elevated RBC count, Hb concentration, PCV, and MCV in a dose-dependent manner, suggesting erythropoietic effects, while MCH and MCHC remained stable. The extract influenced WBC, neutrophil, basophil, lymphocyte, eosinophil, and monocyte counts. Phytochemical analysis identified flavonoids, tannins, saponins, phenolics, and terpenoids, with flavonoids and phenolic compounds being most abundant.
Conclusion: These bioactive compounds may have contributed to the blood parameter changes. The methanol extract of Sabicea brevipes showed significant effects on haematological parameters in Wistar rats, with high safety.

Keywords: Phytoconstituents, Sabicea brevipes, Acute toxicity, Haematological parameters, Erythropoietic effect

Key words: Keywords: Phytoconstituents, Sabicea brevipes, Acute toxicity, Haematological parameters, Erythropoietic effect


 
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How to Cite this Article
Pubmed Style

Ugwu GN, Ugorji CO, Chinwuba P, Onyesife CO, Okonkwo VI, Edeh FC, Odo AO. THE EFFECT OF METHANOL EXTRACT OF Sabicea brevipes (RUBIACEAE) ON SELECTED HAEMATOLOGICAL PARAMETERS IN NORMAL HEALTHY WISTAR RATS. J Exp Lab Med. 2025; 2(3): 127-139. doi:10.5455/JELM.20250504083442

Web Style

Ugwu GN, Ugorji CO, Chinwuba P, Onyesife CO, Okonkwo VI, Edeh FC, Odo AO. THE EFFECT OF METHANOL EXTRACT OF Sabicea brevipes (RUBIACEAE) ON SELECTED HAEMATOLOGICAL PARAMETERS IN NORMAL HEALTHY WISTAR RATS. https://www.wisdomgale.com/jelm/?mno=256062 [Access: June 30, 2025]. doi:10.5455/JELM.20250504083442

AMA (American Medical Association) Style

Ugwu GN, Ugorji CO, Chinwuba P, Onyesife CO, Okonkwo VI, Edeh FC, Odo AO. THE EFFECT OF METHANOL EXTRACT OF Sabicea brevipes (RUBIACEAE) ON SELECTED HAEMATOLOGICAL PARAMETERS IN NORMAL HEALTHY WISTAR RATS. J Exp Lab Med. 2025; 2(3): 127-139. doi:10.5455/JELM.20250504083442


Vancouver/ICMJE Style

Ugwu GN, Ugorji CO, Chinwuba P, Onyesife CO, Okonkwo VI, Edeh FC, Odo AO. THE EFFECT OF METHANOL EXTRACT OF Sabicea brevipes (RUBIACEAE) ON SELECTED HAEMATOLOGICAL PARAMETERS IN NORMAL HEALTHY WISTAR RATS. J Exp Lab Med. (2025), [cited June 30, 2025]; 2(3): 127-139. doi:10.5455/JELM.20250504083442


Harvard Style

Ugwu, G. N., Ugorji, . C. O., Chinwuba, . P., Onyesife, . C. O., Okonkwo, . V. I., Edeh, . F. C. & Odo, . A. O. (2025) THE EFFECT OF METHANOL EXTRACT OF Sabicea brevipes (RUBIACEAE) ON SELECTED HAEMATOLOGICAL PARAMETERS IN NORMAL HEALTHY WISTAR RATS. J Exp Lab Med, 2 (3), 127-139. doi:10.5455/JELM.20250504083442


Turabian Style

Ugwu, Gladys Ndidiamaka, Chimaobi Odinaka Ugorji, Paul Chinwuba, Chioma Obiageli Onyesife, Vivian Ifeoma Okonkwo, Favour Chidiebere Edeh, and Abigail Oluchi Odo. 2025. THE EFFECT OF METHANOL EXTRACT OF Sabicea brevipes (RUBIACEAE) ON SELECTED HAEMATOLOGICAL PARAMETERS IN NORMAL HEALTHY WISTAR RATS. Journal of Experimental and Laboratory Medicine, 2 (3), 127-139. doi:10.5455/JELM.20250504083442


Chicago Style

Ugwu, Gladys Ndidiamaka, Chimaobi Odinaka Ugorji, Paul Chinwuba, Chioma Obiageli Onyesife, Vivian Ifeoma Okonkwo, Favour Chidiebere Edeh, and Abigail Oluchi Odo. "THE EFFECT OF METHANOL EXTRACT OF Sabicea brevipes (RUBIACEAE) ON SELECTED HAEMATOLOGICAL PARAMETERS IN NORMAL HEALTHY WISTAR RATS." Journal of Experimental and Laboratory Medicine 2 (2025), 127-139. doi:10.5455/JELM.20250504083442


MLA (The Modern Language Association) Style

Ugwu, Gladys Ndidiamaka, Chimaobi Odinaka Ugorji, Paul Chinwuba, Chioma Obiageli Onyesife, Vivian Ifeoma Okonkwo, Favour Chidiebere Edeh, and Abigail Oluchi Odo. "THE EFFECT OF METHANOL EXTRACT OF Sabicea brevipes (RUBIACEAE) ON SELECTED HAEMATOLOGICAL PARAMETERS IN NORMAL HEALTHY WISTAR RATS." Journal of Experimental and Laboratory Medicine 2.3 (2025), 127-139. Print. doi:10.5455/JELM.20250504083442


APA (American Psychological Association) Style

Ugwu, G. N., Ugorji, . C. O., Chinwuba, . P., Onyesife, . C. O., Okonkwo, . V. I., Edeh, . F. C. & Odo, . A. O. (2025) THE EFFECT OF METHANOL EXTRACT OF Sabicea brevipes (RUBIACEAE) ON SELECTED HAEMATOLOGICAL PARAMETERS IN NORMAL HEALTHY WISTAR RATS. Journal of Experimental and Laboratory Medicine, 2 (3), 127-139. doi:10.5455/JELM.20250504083442


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