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2025, Vol: 2, Issue: 3
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Original Article
Online Published: 10 Jun 2025
 


Green Tea Extract Mitigates Cigarette Smoke-Induced Sperm Damage in Mus musculus: A Dose-Dependent Study

Aldin Akbar Rahmatullah, Adinda Rizky Trisakti, Syahraini Adhiya Lathifah, Nurrohmah Ratnaningtyas.


Abstract
Aim: This study aimed to evaluate the protective effects of green tea extract (GTE) on sperm concentration and abnormalities in Mus musculus exposed to cigarette smoke, focusing on its dose-dependent antioxidant properties.
Methods: Twenty-five male Mus musculus (12 weeks, 20–25 g) were divided into five groups (n=5): negative control (C-, 0.5 mL 1% Na-CMC, no smoke), positive control (C+, smoke-exposed, 0.5 mL 1% Na-CMC), and three GTE-treated groups (T1, T2, T3; 20, 40, 60 mg/kg body weight, smoke-exposed). GTE was administered daily via oral gavage for 36 days, and smoke exposure involved 20-min sessions with a clove cigarette (2.2-mg nicotine). On day 37, sperm from the cauda epididymis were analyzed for concentration using a hemocytometer and abnormalities via eosin-negrosin staining at 400x magnification. Data were analyzed using one-way ANOVA with Tukey’s post hoc test.
Results: Cigarette smoke significantly reduced sperm concentration in C+ (3.22 ± 0.67 million/mm³) compared to C- (7.15 ± 0.43 million/mm³) and increased abnormalities (59.2 ± 4.21% vs. 13.5 ± 2.90%). GTE dose-dependently improved sperm concentration (T1: 4.37 ± 0.31; T2: 5.61 ± 0.49; T3: 6.87 ± 0.36 million/mm³) and reduced abnormalities (T1: 40.9 ± 3.77%; T2: 32.7 ± 1.80%; T3: 19.4 ± 2.43%), with T3 nearing C- levels (p < 0.05).
Conclusions: GTE, particularly at 60 mg/kg, effectively mitigates cigarette smoke-induced sperm damage in Mus musculus, likely via antioxidant mechanisms, suggesting its potential as a protective agent against reproductive toxicity.

Key words: Green Tea Extract, Male Infertility, Sperm Damage, Tobacco Use


 
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How to Cite this Article
Pubmed Style

Rahmatullah AA, Trisakti AR, Lathifah SA, Ratnaningtyas N. Green Tea Extract Mitigates Cigarette Smoke-Induced Sperm Damage in Mus musculus: A Dose-Dependent Study. J Exp Lab Med. 2025; 2(3): 106-111. doi:10.5455/JELM.20250506015046

Web Style

Rahmatullah AA, Trisakti AR, Lathifah SA, Ratnaningtyas N. Green Tea Extract Mitigates Cigarette Smoke-Induced Sperm Damage in Mus musculus: A Dose-Dependent Study. https://www.wisdomgale.com/jelm/?mno=256501 [Access: June 15, 2025]. doi:10.5455/JELM.20250506015046

AMA (American Medical Association) Style

Rahmatullah AA, Trisakti AR, Lathifah SA, Ratnaningtyas N. Green Tea Extract Mitigates Cigarette Smoke-Induced Sperm Damage in Mus musculus: A Dose-Dependent Study. J Exp Lab Med. 2025; 2(3): 106-111. doi:10.5455/JELM.20250506015046


Vancouver/ICMJE Style

Rahmatullah AA, Trisakti AR, Lathifah SA, Ratnaningtyas N. Green Tea Extract Mitigates Cigarette Smoke-Induced Sperm Damage in Mus musculus: A Dose-Dependent Study. J Exp Lab Med. (2025), [cited June 15, 2025]; 2(3): 106-111. doi:10.5455/JELM.20250506015046


Harvard Style

Rahmatullah, A. A., Trisakti, . A. R., Lathifah, . S. A. & Ratnaningtyas, . N. (2025) Green Tea Extract Mitigates Cigarette Smoke-Induced Sperm Damage in Mus musculus: A Dose-Dependent Study. J Exp Lab Med, 2 (3), 106-111. doi:10.5455/JELM.20250506015046


Turabian Style

Rahmatullah, Aldin Akbar, Adinda Rizky Trisakti, Syahraini Adhiya Lathifah, and Nurrohmah Ratnaningtyas. 2025. Green Tea Extract Mitigates Cigarette Smoke-Induced Sperm Damage in Mus musculus: A Dose-Dependent Study. Journal of Experimental and Laboratory Medicine, 2 (3), 106-111. doi:10.5455/JELM.20250506015046


Chicago Style

Rahmatullah, Aldin Akbar, Adinda Rizky Trisakti, Syahraini Adhiya Lathifah, and Nurrohmah Ratnaningtyas. "Green Tea Extract Mitigates Cigarette Smoke-Induced Sperm Damage in Mus musculus: A Dose-Dependent Study." Journal of Experimental and Laboratory Medicine 2 (2025), 106-111. doi:10.5455/JELM.20250506015046


MLA (The Modern Language Association) Style

Rahmatullah, Aldin Akbar, Adinda Rizky Trisakti, Syahraini Adhiya Lathifah, and Nurrohmah Ratnaningtyas. "Green Tea Extract Mitigates Cigarette Smoke-Induced Sperm Damage in Mus musculus: A Dose-Dependent Study." Journal of Experimental and Laboratory Medicine 2.3 (2025), 106-111. Print. doi:10.5455/JELM.20250506015046


APA (American Psychological Association) Style

Rahmatullah, A. A., Trisakti, . A. R., Lathifah, . S. A. & Ratnaningtyas, . N. (2025) Green Tea Extract Mitigates Cigarette Smoke-Induced Sperm Damage in Mus musculus: A Dose-Dependent Study. Journal of Experimental and Laboratory Medicine, 2 (3), 106-111. doi:10.5455/JELM.20250506015046


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